CELL CULTURE CALCULATOR

Seeding & Subculture

Enter your actual count and culture conditions to calculate the required cell suspension and fresh medium.

Calculation principleReference values are never auto-applied. Routine suspension subculture is calculated as cell-density-based direct dilution.

Cell seeding

Calculate the mix from target cells and final volume per well.

Seeding calculationSingle conditionFixed-decrement series

Concentration input Cell concentrationcells/mL Available suspensionmL Plate Target cells / wellcells Final volume / wellµL Number of wellswells Extra volume%

RESULT

Calculation result

Enter your actual conditions, then select Calculate.